TIME DEPENDENT EFFECT OF THE AQUEOUS SEED EXTRACT OF HELIANTHUS ANNUUS L. ON SOME BIOCHEMICAL PARAMETERS IN ALLOXAN INDUCED – DIABETIC RATS.
TIME DEPENDENT EFFECT OF THE AQUEOUS SEED EXTRACT OF HELIANTHUS ANNUUS L. ON SOME BIOCHEMICAL PARAMETERS IN ALLOXAN INDUCED – DIABETIC RATS.
1Saleh, B.G, & 2 Carol C.D
1Department of integrated Science, College of Education, Gindiri, Plateau State, Nigeria
2 Department of Biochemistry, University of Jos. Plateau State, Nigeria
Email: Carrll42@yahoo.com, bitrussaleh@gmail.com
Abstract
The study was carried out to investigate the effect of the aqueous extract of helianthus annuus L seed and time of treatment on serum glucose, protein, urea, albumin, creatinine and some electrolytes using Wista albino rats as the animal model. Twenty (20) adult male wista albino rats were randomly assigned to four (4) groups of five (5) rats each. Diabetes was induced in the test and control rats by the intraperitoneal injection of alloxan monohydrate at a dose of 150mg/kg b. w. The experimental rats were administered 400mg/kg b. w single daily dose of the extract for fifteen (15) days. Diabetes was confirmed in the experimental rats by the significant increase (p<0.05) in serum glucose level. Treatment with helianthus annuus seed extract showed a significant time dependant decrease (p<0.05) in serum glucose level. The diabetic rats showed significant (p<0.05) increase in the serum levels of urea, creatinine. Treatment with the extract resulted in a time dependent significant (p<0.05) decrease in these parameters. There was a significant (p<0.05) decrease in the serum levels of total protein and albumin. Treatment with extract produced significant (p<0.05) increase in these parameters. This investigation also showed a significant (p<0.05) decrease in serum levels of Na+, K+, Ca+, Mg2+ and Cl– in the diabetic rats. Treatment with the extract produced significant (p<0.05) time dependent increase in the levels of these electrolytes.
Keywords: Helianthus Annuus L, Diabetes Mellitus, Glucose, Electrolytes